Weak mitochondrial targeting sequence determines tissue ...
The mitochondrial pellet was washed twice at 12,000 g and resuspended in HIM. The cytoplasmic supernatant was centrifuged at 120,000 g for 60 minutes and the pellet was discarded. Total protein extract was obtained by homogenization in Passive Lysis Buffer (Promega) by five strokes with pestle A and 20 strokes with pestle B, sonication for 5 ...
